Demineralized Bone Matrix Incorporated PLGA Matrices
Poly(lactic-co-glycolic acid)-PLGA (85:15) films incorporated with demineralized bone matrix (DBM) powder with the weight ratio of polymer: DBM (75:25) were investigated for release of agents including osteoinductive (OI) factors during an 80 day period exposed to Phosphate Buffered Saline (PBS) at 37 °C. The release amounts of agents including OI factors from DBM/PLGA matrices were more than 2-fold higher at 70 days than at 10 days, respectively. This result demonstrates that controlled release of OI factors can be achieved for extended time period at a target site using PLGA as a carrier for DBM powder. Murine Bone Marrow Stromal Cell (BMSC) attachment was studied with different time points at 30 min, 1 h, 2 h, 4 h, 6 h and 24 h for DBM/PLGA and PLGA control matrices. Significantly higher number of BMSCs attached to the DBM/PLGA matrices at each time point compared to the controls. This result suggests that BMSCs favorably attach to surfaces having OI properties. If DBM is incorporated into bioegradable 3-D polymer scaffolds and cultured with BMSCs, those scaffolds could potentially be used for bone tissue engineering applications.