Breast (MCF-7) and Melanoma (A-375) Cancer Cell Lines Treated with Cryptantha Cinerea and Cryptantha Flava in Agarose Culture
Two plant species of the family Boraginaceae, Crypthantha cinerea and Crypthantha flava, were used by various Native American tribes for different medicinal purposes. The Hopi tribe used C. flava as an anti-cancer treatment. The purpose of this study was to examine the effect of C. flava and C. cinerea on breast (MCF-7) and melanoma (A-375) cancer cell lines. Cancer cells were first grown in monolayer, removed at confluence, and cultured in the three-dimensional growth system for up to 1.5 weeks. At the end of the growth period, each treatment group was analyzed for cell viability and mitosis. Student T-tests assuming unequal variances were used for statistical analysis. All treatments were compared to the vehicle control. MCF-7 cells treated with C. flava showed a significant (p<0.05) decrease in mitotic activity with treatments of 400 µg/mL and 800 µg/mL. A-375 cells treated with C. flava showed a significant (p<0.05) decrease in mitosis and cell viability at 200 µg/mL and higher. For both MCF-7 and A-375 cells treated with C. cinerea there was a significant (p<0.05) decrease in both mitosis and cell viability at all doses. MCF-7 cells treated with C. cinerea showed evidence of apoptosis when stained with Hoescht and Propidium Iodide.